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KMID : 0382619880080020719
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Hanyang Journal of Medicine
1988 Volume.8 No. 2 p.719 ~ p.731
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Ultrastructure and Blood-Iris Barrier in Experimental Rubeosis Iridis in Rabbit
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Abstract
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Iris neovascularization was produced in rabbit by hypotony following aspiration of the vitreous. The hypotony was produced after 0.3m1 of vitreous fluid was aspirated using 25 gauge needle through the pars Plana of the 10 rabbits. The rubeosis iris di was confirmed under the slit-lamp examination.
For the histochemical study, horseradish peroxidase was injected through the earlobe vein. After fixation of the iris, the tissue was treated with diaminobenzidine and examined with both light microscopy and transmission electron microscopy.
The newly formed vessel was abundant particularly on the upper stroma of the iris. The new vessel,formation was evident from the proliferation of the endothelial cell which may be derived from preexisting iris vessels. The endothelial cells of the newly formed vessels revealed prominent vinous processes into the vascular lumen, formation of the marginal flap and numerous fenestrations in the endothelial cell junction. The horseradish peroxidase was apparent through the endothelial junction into the extravascular space by the cytochemical electron microscope.
These results suggest that hypotony in the rabbit produces the disruption of the blood-iris barrier and this model may contribute to the formation of the iris neovascularization.
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